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pretreatment module  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pretreatment module
    Pretreatment Module, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pretreatment+module/pretreatment+module/10__22203_slash_ecm__v033a11-128-16-18
    Average 90 stars, based on 1 article reviews
    pretreatment module - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection
    Article Snippet: .. The sections were subjected to heatinduced epitope retrieval (HIER) (van der Loos, 2010), performed in a PreTreatment Module (Lab Vision) by incubation in citrate buffer pH 6.0 (TA-250-PM1X, Thermo Fisher Scientific) for 20 min at 98 °C, rinsed with tap water and incubated with Superblock (AAA999, Klinipath) for 10 min at RT. .. Sections were incubated overnight at 4 °C with rat anti-mouse monoclonal F4/80 (macrophages; MCA497GA, clone: CI:A3-1, Serotec) diluted 1: 1000 in Tris-HCl-buffered saline (TBS; 50 mM Tris, 0.9 % NaCl), subsequently with rabbit anti-rat IgG (6130-01, fab2; SBA/ITK) diluted 1: 3000 in TBS with 20 % normal mouse serum for 30 min at RT, followed by undiluted BrightVision anti-rabbit AP (DPVB-55AP, Immunologic) for 30 min at RT and finally with Vector Blue (SK-5300, Vector Labs) for 10 min at RT, with three TBS washes between all incubations.

    Concentration Assay:

    Article Title: The expression and prognostic value of toll-like receptors (TLRs) in pancreatic cancer patients treated with neoadjuvant therapy
    Article Snippet: .. After xylene deparaffinization and rehydration through a gradually decreasing concentration of ethanol to distilled water, the slides were treated in a PreTreatment module (Lab Vision Corp, Fremont, CA, USA) in a Tris-HCl pre-treatment buffer (pH 8.5) with TLRs 1, 2 and 4 and in Tris-EDTA (pH 9.0) with TLRs 3, 5, 7, and 9 for 20 min at 98°C for antigen retrieval. .. For the staining procedure, an Autostainer 480 (Lab Vision) was used.

    Article Title: Elevated tumor expression of Astroprincin (FAM171A1) is an independent marker of poor prognosis in colon cancer
    Article Snippet: .. The tumor tissue microarray (TMA) blocks were freshly cut into 4-μm sections, fixed on slides, and dried at 37 °C for 12 to 24 h. Then continued by deparaffinization in xylene and rehydration through a gradually decreasing concentration of ethanol to distilled water, TMA-slides were treated in a PreTreatment module (Lab Vision Corp., Fremont, CA, USA) in Tris-HCL buffer for 20 min at 98 °C for antigen retrieval. .. We stained sections with Autostainer 480 (LabVision) using Dako REAL EnVision Detection System, Peroxidase/DAB+, Rabbit/Mouse (Dako, Glostrup, Denmark).

    Microarray:

    Article Title: Elevated tumor expression of Astroprincin (FAM171A1) is an independent marker of poor prognosis in colon cancer
    Article Snippet: .. The tumor tissue microarray (TMA) blocks were freshly cut into 4-μm sections, fixed on slides, and dried at 37 °C for 12 to 24 h. Then continued by deparaffinization in xylene and rehydration through a gradually decreasing concentration of ethanol to distilled water, TMA-slides were treated in a PreTreatment module (Lab Vision Corp., Fremont, CA, USA) in Tris-HCL buffer for 20 min at 98 °C for antigen retrieval. .. We stained sections with Autostainer 480 (LabVision) using Dako REAL EnVision Detection System, Peroxidase/DAB+, Rabbit/Mouse (Dako, Glostrup, Denmark).



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